C19orf35 Mouse Monoclonal Antibody [3-B7]
Catalog# M1506-5
C19orf35 Mouse Monoclonal Antibody [3-B7]
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
C19orf35 Mouse Monoclonal Antibody [3-B7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human C19orf35 aa 1-473 / 473.
Species Reactivity
Human, Mouse
Validated Applications
IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Human testis tissue, Hela, hES cells
Conjugation
unconjugated
Clone Number
3-B7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:200-1:500
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IF-Cell
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1:200
Target
Function
Probable catalytically inactive kinase (Probable). Interacts with CRK-II and antagonizes CRK-II-signaling. Prevents the formation of CRK-II-dependent membrane ruffling and lamellipodia-like extensions 1.
Background References
1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004)
Sequence Similarity
Belongs to the protein kinase superfamily.
Subcellular Location
Actin cytoskeleton, focal adhesion.
UNIPROT
Synonyms
C19orf35 antibody
chromosome 19 open reading frame 35 antibody
CS035_HUMAN antibody
FLJ45778 antibody
Putative uncharacterized protein C19orf35 antibody
Images
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Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-C19orf35 antibody (M1506-5) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1506-5) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
ICC staining C19orf35 in human embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C19orf35 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"