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C19orf35 Mouse Monoclonal Antibody [3-B7]

Usd: 350

Specification

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Catalog# M1506-5

C19orf35 Mouse Monoclonal Antibody [3-B7]

Application

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

C19orf35 Mouse Monoclonal Antibody [3-B7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human C19orf35 aa 1-473 / 473.

Species Reactivity

Human, Mouse

Validated Applications

IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

Human testis tissue, Hela, hES cells

Conjugation

unconjugated

Clone Number

3-B7

RRID

AB_3073115

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:500

  • IF-Cell

  • 1:200

Target

Function

Probable catalytically inactive kinase (Probable). Interacts with CRK-II and antagonizes CRK-II-signaling. Prevents the formation of CRK-II-dependent membrane ruffling and lamellipodia-like extensions 1.

Background References

1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004)

Sequence Similarity

Belongs to the protein kinase superfamily.

Subcellular Location

Actin cytoskeleton, focal adhesion.

UNIPROT

SwissProt: Q6ZS72 Human

Synonyms

C19orf35 antibody

chromosome 19 open reading frame 35 antibody

CS035_HUMAN antibody

FLJ45778 antibody

Putative uncharacterized protein C19orf35 antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-C19orf35 antibody (<a href="/products/M1506-5" style="font-weight: bold;text-decoration: underline;">M1506-5</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1506-5" style="font-weight: bold;text-decoration: underline;">M1506-5</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-C19orf35 antibody (M1506-5) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1506-5) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining C19orf35 in human embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining C19orf35 in human embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining C19orf35 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining C19orf35 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"