VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
Catalog# M1510-10
VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
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WB
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IP
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ELISA
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Species independent
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unconjugated
Overview
Product Name
VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide (YTDIEMNRLGKC).
Species Reactivity
Species independent
Validated Applications
WB, IP, ELISA
Positive Control
Recombinant VSV-G-tag protein.
Conjugation
unconjugated
Clone Number
E0-G7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:20,000
Target
Function
Vesicular Stomatitis Virus (VSV) is an arbovirus in the family Rhabdoviridae, order Mononegavirales. VSV infects insects and mammals. The genome of VSV is a negative-sense RNA strand that encodes five major proteins: glycoprotein (G), matrix protein (M), nucleoprotein (NC), large protein (L) and phosphoprotein. The L protein and the phosphoprotein combine to catalyze the replication of VSV’s mRNA. After endocytosis, the G protein facilitates VSV’s entry into the cell by controlling virus attachment to the host cell as well as fusion of the viral envelope with the endosomal membrane. Transport of the G protein from the endoplasmic reticulum (ER) to the plasma membrane (PM) is temperature sensitive. Because of this property, VSV is commonly used in research labs to study the properties of viruses in the Rhabdoviridae family, and to study viral evolution.
Background References
1. Niemel EH et al. Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome. Nucleic Acids Res 42:7358-69 (2014).
2. J ger W et al. Ultrasound-guided intramural inoculation of orthotopic bladder cancer xenografts: a novel high-precision approach. PLoS One 8:e59536 (2013).
Synonyms
Vesicular stomatitis virus glycoprotein tag antibody
VSV epitope tag antibody
VSV tag antibody
VSV-G epitope tag antibody
VSVG antibody
vsvg tag antibody
Images
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Western blot analysis of VSV-G-tag on recombinant VSV-G-tag protein with Mouse anti-VSV-G-tag antibody (M1510-10) at different dilutions.
Lysates/proteins at 50 ng/Lane.
Exposure time: 15 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-10) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"