human IgM Mouse Monoclonal Antibody [B7-3]
Catalog# M1510-5
human IgM Mouse Monoclonal Antibody [B7-3]
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WB
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IHC-P
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ELISA
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Human
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unconjugated
Overview
Product Name
human IgM Mouse Monoclonal Antibody [B7-3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
native protein.
Species Reactivity
Human
Validated Applications
WB, IHC-P, ELISA
Target Molecular Weight
50 KD
Positive Control
Recombinant proteint lysates, human liver tissue, mouse spleen tissue.
Conjugation
unconjugated
Clone Number
B7-3
RRID
Product Features
Form
Liquid
Concentration
2 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:200-1:500
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IHC-P
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1:50-1:100
Target
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions.
Background References
1. Tisch R.et.al.Functional differences between immunoglobulins M and D expressed on the surface of an immature B-cell line.Proc. Natl. Acad. Sci. U.S.A. 85:6914-6918(1988).
Subcellular Location
Cell membrane, Immunoglobulin, Membrane, Secreted.
UNIPROT
Synonyms
AGM1 antibody
Constant region of heavy chain of IgM antibody
DKFZp686I15196 antibody
DKFZp686I15212 antibody
FLJ00385 antibody
Hepatitis B virus receptor binding protein antibody
Ig mu chain C region antibody
IGHM antibody
IgM heavy chain constant region antibody
Immunoglobulin heavy constant mu antibody
ExpandImages
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Western blot analysis of human IgM on recombinant proteint lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-human IgM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-human IgM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-5, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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