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Mesothelin Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-76

Mesothelin Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Mesothelin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human Mesothelin aa 220-410.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 69 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH:OVCAR8 (Human ovarian adenocarcinoma cell) cell lysate, SiHa (Human cervical cancersSquamous cell) cell lysate, NCI-H226 (Human pleural mesothelioma cell) cell lysate, human tonsil tissue, human lung cancer tissue.

Conjugation

unconjugated

RRID

AB_3069271

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:50-1:200

Target

Function

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. Mesothelin is over expressed in several human tumors, including mesothelioma, ovarian cancer, pancreatic adenocarcinoma and cholangiocarcinoma. The interaction between mesothelin and MUC16 (also known as CA125) may facilitate the implantation and peritoneal spread of tumors by cell adhesion. Dr. Mitchell Ho and colleagues at the National Cancer Institute identified the region (residues 296-359) consisting of 64 amino acids at the N-terminus of cell surface mesothelin as the functional binding domain for MUC16.

Background References

1. Ho M et al. Humoral immune response to mesothelin in mesothelioma and ovarian cancer patients. Clin Cancer Res 11:3814-3820 (2005).

2. Kojima T et al. Molecular cloning and expression of megakaryocyte potentiating factor cDNA. J Biol Chem 270:21984-21990 (1995).

Sequence Similarity

Belongs to the mesothelin family.

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Post-translational Modification

Both MPF and the cleaved form of mesothelin are N-glycosylated.; Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

Subcellular Location

Cell membrane, Golgi apparatus, Membrane, Secreted.

UNIPROT

SwissProt: Q13421 Human

Synonyms

CAK 1 antibody

CAK1 antibody

CAK1 antigen antibody

cleaved form antibody

Megakaryocyte potentiating factor antibody

Mesothelin antibody

Mesothelin isoform 1 precursor antibody

MPF antibody

Msln antibody

MSLN_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (<a href="/products/ER1803-76" style="font-weight: bold;text-decoration: underline;">ER1803-76</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: NIH:OVCAR8 (Human ovarian adenocarcinoma cell) cell lysate<br />Lane 3: PC-3 (Human prostate cancer cell) cell lysate<br />Lane 4: SiHa (Human cervical cancersSquamous cell) cell lysate<br />Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 6: NCI-H226 (Human pleural mesothelioma cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 16 seconds; ECL: K1801<br /><br />PC-3 is a negative control (PMID: 27760836).<br />A549 is a low expression (PMID: 25118887).<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-76" style="font-weight: bold;text-decoration: underline;">ER1803-76</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 69 kDa<br />Observed band size: 45 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (ER1803-76) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: NIH:OVCAR8 (Human ovarian adenocarcinoma cell) cell lysate
    Lane 3: PC-3 (Human prostate cancer cell) cell lysate
    Lane 4: SiHa (Human cervical cancersSquamous cell) cell lysate
    Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 6: NCI-H226 (Human pleural mesothelioma cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 16 seconds; ECL: K1801

    PC-3 is a negative control (PMID: 27760836).
    A549 is a low expression (PMID: 25118887).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-76, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 69 kDa
    Observed band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-76" style="font-weight: bold;text-decoration: underline;">ER1803-76</a>) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody (<a href="/products/ER1803-76" style="font-weight: bold;text-decoration: underline;">ER1803-76</a>) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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