HRP Conjugated Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-76] - Detector
Overview
Product Name
HRP Conjugated Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-76] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Apolipoprotein B aa 401-810 (HA211327).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327).
Conjugation
HRP
Clone Number
PSH17-76
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-75] to Mouse Apolipoprotein B/Apo B2 antibody (Capture) (HA723944) or Rabbit monoclonal [PSH17-77] to Mouse Apolipoprotein B/Apo B2 antibody (Capture) (HA723946) and Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) as the standard. The reference range value is 625-20,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. Its measurement is commonly used to detect the risk of atherosclerotic cardiovascular disease. The protein occurs in the plasma in two main isoforms, ApoB48 and ApoB100. The first is synthesized exclusively by the small intestine, the second by the liver. ApoB-100 is the largest of the apoB group of proteins, consisting of 4563 amino acids, including a 27-amino acid signal peptide and a 4536-amino acid mature protein. Both isoforms are coded by APOB and by a single mRNA transcript larger than 16 kb. ApoB48 is generated when a stop codon (UAA) at residue 2153 is created by RNA editing. There appears to be a trans-acting tissue-specific splicing gene that determines which isoform is ultimately produced.[citation needed] Alternatively, there is some evidence that a cis-acting element several thousand bp upstream determines which isoform is produced. As a result of the RNA editing, ApoB48 and ApoB100 share a common N-terminal sequence, but ApoB48 lacks ApoB100's C-terminal LDL receptor binding region. ApoB48 is named because it constitutes 48% of the sequence for ApoB100. ApoB48 is a unique protein in chylomicrons from the small intestine. After most of the lipids in the chylomicron have been absorbed, ApoB48 returns to the liver as part of the chylomicron remnant, where it is endocytosed and degraded.
Background References
1. De Oliveira-Gomes D et al. Apolipoprotein B: Bridging the Gap Between Evidence and Clinical Practice. Circulation. 2024 Jul
2. Contois JH et al. Standardization of Apolipoprotein B, LDL-Cholesterol, and Non-HDL-Cholesterol. J Am Heart Assoc. 2023 Aug
Subcellular Location
Cytoplasm, Secreted, Lipid droplet.
UNIPROT
Synonyms
Apo B 100 antibody
Apo B antibody
Apo B-100 antibody
Apo B-48 antibody
Apo B100 antibody
Apo B48 antibody
ApoB 100 antibody
ApoB 48 antibody
APOB antibody
Apolipoprotein B (including Ag(x) antigen) antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse Apolipoprotein B/Apo B matched pair antibodies<br /><br />Capture: <a href="/products/HA723944" style="font-weight: bold;text-decoration: underline;">HA723944</a>, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75]<br />Detector: <a href="/products/HA723945" style="font-weight: bold;text-decoration: underline;">HA723945</a>, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723946" style="font-weight: bold;text-decoration: underline;">HA723946</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein B/Apo B protein (<a href="/products/HA211327" style="font-weight: bold;text-decoration: underline;">HA211327</a>) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723945" style="font-weight: bold;text-decoration: underline;">HA723945</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723945H_1.jpg?v=20260401002658)
Sandwich ELISA analysis of Mouse Apolipoprotein B/Apo B matched pair antibodies
Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723946) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723945, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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