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HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody

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Catalog# NBI02H

HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody

Application

  • WB

  • IP

Reactivity

  • Mouse

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody

Antibody Type

Alpaca Nanobody

Immunogen

Mouse IgG

Species Reactivity

Mouse

Validated Applications

WB, IP

Conjugation

HRP

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at –20 ℃, protect from light.

Storage Buffer

PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol.

Isotype

IgG

Purification Method

Recombinant Expression and Affinity purified,Dissociation constant KD of 0.1nM.

Application Dilution

  • WB

  • 1:1,000-1:10,000

  • IP

  • sample should be completely reduced/denatured before loaded onto a western blot.

Target

Function

Anti-mouse IgG Nano-secondary antibody for IP (HRP) is based on Monovalent, recombinant single domain antibodies to mouse IgG coupled to HRP, and the Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protien was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protien. Using Anti-mouse IgG for IP Nano-secondary antibody (HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.

Background References

暂无

Images

  • Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(<a href="/products/M1403-2" style="font-weight: bold;text-decoration: underline;">M1403-2</a>) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) (1/1000) was used to confirm successful immunoprecipation.<br />Lane1/2: Immunoprecipitating IgG in recombinant protein with flag on N-terminal<br />Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (<a href="/products/M1403-2" style="font-weight: bold;text-decoration: underline;">M1403-2</a>) in recombinant protein with flag on N-terminal

    Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation.
    Lane1/2: Immunoprecipitating IgG in recombinant protein with flag on N-terminal
    Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with flag on N-terminal

  • PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> IP in HeLa cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 24 seconds; ECL: K1801

    PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA601172 IP in HeLa cell lysate
    Lane 3: Mouse IgG instead of HA601172 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 24 seconds; ECL: K1801

  • PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: NIH/3T3 cell lysate (input)<br />Lane 2: <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> IP in NIH/3T3 cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA601172" style="font-weight: bold;text-decoration: underline;">HA601172</a> in NIH/3T3 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 5 seconds; ECL: K1802

    PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: NIH/3T3 cell lysate (input)
    Lane 2: HA601172 IP in NIH/3T3 cell lysate
    Lane 3: Mouse IgG instead of HA601172 in NIH/3T3 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 5 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation