NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44] - BSA and Azide free
Catalog# HA751003
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44] - BSA and Azide free
-
WB
-
Human
-
HA722282
含抗保成分
-
unconjugated
Overview
Product Name
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NCOA4 aa 1-400 / 614.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 70 kDa
Positive Control
HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate.
Conjugation
unconjugated
Clone Number
PSH05-44
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
Target
Function
Nuclear receptor coactivator 4, also known as Androgen Receptor Activator (ARA70), is a protein that in humans is encoded by the NCOA4 gene. It plays an important role in ferritinophagy, acting as a cargo receptor, binding to the ferritin heavy chain and latching on to ATG8 on the surface of the autophagosome.
Background References
1. Santana-Codina N et al. The Role of NCOA4-Mediated Ferritinophagy in Ferroptosis. Adv Exp Med Biol. 2021
2. Wu H et al. ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4. Autophagy. 2023 Jul
Subcellular Location
Nucleus.
UNIPROT
Synonyms
70 kDa androgen receptor activator antibody
70 kDa androgen receptor coactivator antibody
70 kDa AR activator antibody
70 kDa AR-activator antibody
Androgen receptor coactivator 70 kD antibody
Androgen receptor coactivator 70 kDa protein antibody
Androgen receptor-associated protein of 70 kDa antibody
ARA 70 antibody
ARA70 antibody
DKFZp762E1112 antibody
ExpandImages
-
Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA751003) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A-172 cell lysate
Lane 3: A549 cell lysate
Lane 4: A431 cell lysate
Lane 5: HL-60 cell lysate
Lane 6: NIH:OVCAR-3 cell lysate
Lane 7: A375 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751003) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA751003) at 1/5,000 dilution.
Lane 1: KYSE150-si NT cell lysate
Lane 2: KYSE150-si NCOA4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751003) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]
Application: IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45] - BSA and Azide free
Application: IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NCOA4 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
