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NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]

Usd: 385

Specification

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Catalog# HA722282

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NCOA4 aa 1-400 / 614.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 70 kDa

Positive Control

HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate.

Conjugation

unconjugated

Clone Number

PSH05-44

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

Target

Function

Nuclear receptor coactivator 4, also known as Androgen Receptor Activator (ARA70), is a protein that in humans is encoded by the NCOA4 gene. It plays an important role in ferritinophagy, acting as a cargo receptor, binding to the ferritin heavy chain and latching on to ATG8 on the surface of the autophagosome.

Background References

1. Santana-Codina N et al. The Role of NCOA4-Mediated Ferritinophagy in Ferroptosis. Adv Exp Med Biol. 2021

2. Wu H et al. ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4. Autophagy. 2023 Jul

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q13772 Human

Synonyms

70 kDa androgen receptor activator antibody

70 kDa androgen receptor coactivator antibody

70 kDa AR activator antibody

70 kDa AR-activator antibody

Androgen receptor coactivator 70 kD antibody

Androgen receptor coactivator 70 kDa protein antibody

Androgen receptor-associated protein of 70 kDa antibody

ARA 70 antibody

ARA70 antibody

DKFZp762E1112 antibody

Expand

Images

  • Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (<a href="/products/HA722282" style="font-weight: bold;text-decoration: underline;">HA722282</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A-172 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: A431 cell lysate<br />Lane 5: HL-60 cell lysate<br />Lane 6: NIH:OVCAR-3 cell lysate<br />Lane 7: A375 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 70 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722282" style="font-weight: bold;text-decoration: underline;">HA722282</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA722282) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A-172 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: A431 cell lysate
    Lane 5: HL-60 cell lysate
    Lane 6: NIH:OVCAR-3 cell lysate
    Lane 7: A375 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 70 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (<a href="/products/HA722282" style="font-weight: bold;text-decoration: underline;">HA722282</a>) at 1/5,000 dilution.<br /><br />Lane 1: KYSE150-si NT cell lysate<br />Lane 2: KYSE150-si NCOA4 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 70 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722282" style="font-weight: bold;text-decoration: underline;">HA722282</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA722282) at 1/5,000 dilution.

    Lane 1: KYSE150-si NT cell lysate
    Lane 2: KYSE150-si NCOA4 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 70 kDa
    Observed band size: 75 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722282) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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