iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Catalog# HA720178F
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
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IF-Cell
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FC
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Human
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Mouse
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NSE aa 30-70.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Cell, FC
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
SH-SY5Y.
Conjugation
iFluor™ 488
Clone Number
SC06-28
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:50
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FC
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1:500-1:1,000
Target
Function
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Background References
1. Chang BS et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015).
2. Sun Y et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014).
Sequence Similarity
Belongs to the enolase family.
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Subcellular Location
Cytoplasm, Cell membrane.
Synonyms
2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma, neuronal) antibody
Enolase 2 antibody
Enolase 2 gamma neuronal antibody
Enolase2 antibody
ExpandImages
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Immunocytochemistry analysis of SH-SY5Y cells labeling NSE with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of SH-SY5Y cells labeling NSE.
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with NSE (HA720178F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: WB
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
