NSUN4 Mouse Monoclonal Antibody [4G4]
Catalog# HA600023
NSUN4 Mouse Monoclonal Antibody [4G4]
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WB
-
IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
NSUN4 Mouse Monoclonal Antibody [4G4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human NSUN4 aa 1-384.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, mouse testis tissue, human prostate tissue.
Conjugation
unconjugated
Clone Number
4G4
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
-
1:500-1:1,000
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IHC-P
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1:100-1:500
Target
Function
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Background References
1. Camara Y. et. al. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13:527-539(2011).
Subcellular Location
Mitochondrion.
Synonyms
5 methylcytosine rRNA methyltransferase NSUN4 antibody
EC=2.1.1.- antibody
NOL1/NOP2/Sun domain family member 4 antibody
NOL1/NOP2/Sun domain family, member 4 antibody
NOP2/Sun domain family, member 4 antibody
Nsun4 antibody
NSUN4_HUMAN antibody
Putative methyltransferase NSUN4 antibody
RP4-603I14.2 antibody
Shtap antibody
ExpandImages
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Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600023) at 1/500 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HeLa cell lysate
Lane 6: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600023) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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