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NeuN Recombinant Antibody [SR45-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610237

NeuN Recombinant Antibody [SR45-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

NeuN Recombinant Antibody [SR45-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human NeuN aa 20-60.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

SR45-07

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:1,000

  • IHC-P

  • 1:2,000

Target

Function

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Background References

1. Santamaría G et al. NeuN distribution in brain structures of normal and Zika-infected suckling mice. J Mol Histol. 2023 Jun

2. Luijerink L et al. Immunostaining for NeuN Does Not Show all Mature and Healthy Neurons in the Human and Pig Brain: Focus on the Hippocampus. Appl Immunohistochem Mol Morphol. 2021 Jul

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene: 143966 Rat

Synonyms

FLJ56884 antibody

FLJ58356 antibody

Fox-1 homolog C antibody

fox1 homolog C antibody

Fox3 antibody

FOX3NeuN antibody

hexaribonucleotide binding protein 3 antibody

HRNBP3 antibody

NEUN antibody

neuronal nuclei antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000 (NeuN, <a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>, Rat, green); 1/500 (VGLUT1, <a href="/products/HA723217" style="font-weight: bold;text-decoration: underline;">HA723217</a>, Rabbit, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/1,000 (NeuN, HA610237, Rat, green); 1/500 (VGLUT1, HA723217, Rabbit, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NeuN antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NeuN antibody (HA610237) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610237) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NeuN antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NeuN antibody (HA610237) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610237) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NeuN antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610237" style="font-weight: bold;text-decoration: underline;">HA610237</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NeuN antibody (HA610237) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610237) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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