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NeuN Recombinant Antibody [SR45-07] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610316

NeuN Recombinant Antibody [SR45-07] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

NeuN Recombinant Antibody [SR45-07] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human NeuN aa 20-60.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Human brain tissue, mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

SR45-07

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:1,000

  • IHC-P

  • 1:5,000

  • WB

  • 1:10,000

Target

Function

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Background References

1. Santamaría G et al. NeuN distribution in brain structures of normal and Zika-infected suckling mice. J Mol Histol. 2023 Jun

2. Luijerink L et al. Immunostaining for NeuN Does Not Show all Mature and Healthy Neurons in the Human and Pig Brain: Focus on the Hippocampus. Appl Immunohistochem Mol Morphol. 2021 Jul

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene: 143966 Rat

Synonyms

FLJ56884 antibody

FLJ58356 antibody

Fox-1 homolog C antibody

fox1 homolog C antibody

Fox3 antibody

FOX3NeuN antibody

hexaribonucleotide binding protein 3 antibody

HRNBP3 antibody

NEUN antibody

neuronal nuclei antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000 (NeuN, <a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>, Mouse, green); 1/200 (QKI-6, <a href="/products/HA601502" style="font-weight: bold;text-decoration: underline;">HA601502</a>, Guinea pig, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/1,000 (NeuN, HA610316, Mouse, green); 1/200 (QKI-6, HA601502, Guinea pig, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (HA610316) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610316) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (HA610316) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610316) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (HA610316) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610316) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/10,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: C2C12 cell lysate (negative)<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 45/50 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610316" style="font-weight: bold;text-decoration: underline;">HA610316</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (HA610316) at 1/10,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: C2C12 cell lysate (negative)
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 45/50 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610316) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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