Olig2 Recombinant Antibody [SP07-02] - Mouse IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610427
Olig2 Recombinant Antibody [SP07-02] - Mouse IgG1 (Chimeric) - BSA and Azide free
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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HA601629
含抗保成分
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unconjugated
Overview
Product Name
Olig2 Recombinant Antibody [SP07-02] - Mouse IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human Olig2 aa 238-287 / 323.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 32 kDa
Conjugation
unconjugated
Clone Number
SP07-02
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:100
Target
Function
The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG2 is more widely expressed in embryonic brain than OLIG1, while OLIG3 is primarily expressed in non-neural tissues. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.
Background References
1. Kiely AP et al. a-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson\'s disease and multiple system atrophy Acta Neuropathol 125:753-69 (2013).
2. Wang K et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009).
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
Basic domain helix loop helix protein class B 1 antibody
Basic helix loop helix protein class B 1 antibody
BHLHB antibody
bHLHB1 antibody
bHLHe19 antibody
Class B basic helix loop helix protein 1 antibody
Class B basic helix-loop-helix protein 1 antibody
class E basic helix loop helix protein 19 antibody
Class E basic helix-loop-helix protein 19 antibody
Human protein kinase C binding protein RACK17 antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610427, 1/200, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Embryo
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610427, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Embryo(brain)
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610427, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610427, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610427, 1/200, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA610427, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA610427, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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