Osteopontin Rabbit Polyclonal Antibody
Catalog# HA500464
Osteopontin Rabbit Polyclonal Antibody
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WB
-
FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Osteopontin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse steopontin aa 1-50 / 314.
Species Reactivity
Human, Mouse
Validated Applications
WB, FC
Molecular Weight
32 kDa
Positive Control
Mouse liver tissue lysate, HepG2 cell lysate, 213.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
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FC
-
1:50-1:100
Target
Function
Osteopontin is an extracellular matrix cell adhesion protein which is abundant in bone and synthesized by preosteoblasts, osteoblasts and osteoclastic cells that are localized in the mineralized phase of bone matrix. Osteopontin probably exists in tissue-specific isoforms that may correspond to particular cellular functions. Acts as a cytokine it involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Background References
1. Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;"Large-scale phosphorylation analysis of mouse liver.";Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
2. Ashkar S., Weber G.F., Panoutsakopoulou V., Sanchirico M.E., Jansson M., Zawaideh S., Rittling S.R., Denhardt D.T., Glimcher M.J., Cantor H.;"Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity.";Science 287:860-864(2000).
Subcellular Location
Secreted.
Synonyms
BNSP antibody
Bone sialoprotein 1 antibody
BSP I antibody
BSPI antibody
Early T lymphocyte activation 1 antibody
ETA 1 antibody
ETA1 antibody
MGC110940 antibody
Nephropontin antibody
OPN antibody
ExpandImages
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Western blot analysis of Osteopontin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500464, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse liver tissue lysate
Lane 2: HepG2 cell lysate -
Flow cytometric analysis of Osteopontin was done on 213 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500464, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
SPP1 as a key modulator of M2 macrophage polarization promotes endometriosis progression via activation of the FAK/PI3K/AKT pathway: A bioinformatics and experimental study
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.115563
IF: 4.7
Application: WB
Reactivity: Mouse,Human
Publish date: 2025 Sept
-
Dynamically programmable plant-derived scaffold coordinates alveolar bone repair cascade: Phase-adaptive integration from hemostasis to bone remodeling
Journal: Chemical Engineering Journal
DOI: 10.1016/j.cej.2025.168416
IF: 13.2
Application: WB
Reactivity: Mouse
Publish date: 2025 Sept
-
Genome editing of Spp1 by inhalable CRISPR/Cas9 formulation for treating pulmonary fibrosis
Journal: Journal Of Controlled Release
DOI: 10.1016/j.jconrel.2025.114424
IF: 11.5
Application:
Reactivity:
Publish date: 2025 Nov
-
LATS1/YAP1 Axis Controls Bone Regeneration on Distraction Osteogenesis by Activating Wnt/β-catenin
Journal: Tissue Engineering Part A
DOI:
IF: 4.1
Application: WB
Reactivity: Rat
Publish date: 2023 Nov
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