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Osteopontin Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0806-6

Osteopontin Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

Predicted reactivity

  • Human

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Osteopontin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Osteopontin aa 1-314.

Species Reactivity

Mouse (Predicted: Human, Rat)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 35 kDa

Positive Control

RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate.

Conjugation

unconjugated

RRID

AB_3068666

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.

Background References

1. Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;"Large-scale phosphorylation analysis of mouse liver.";Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).

2. Ashkar S., Weber G.F., Panoutsakopoulou V., Sanchirico M.E., Jansson M., Zawaideh S., Rittling S.R., Denhardt D.T., Glimcher M.J., Cantor H.;"Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity.";Science 287:860-864(2000).

Sequence Similarity

Belongs to the osteopontin family.

Post-translational Modification

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

Synonyms

BNSP antibody

Bone sialoprotein 1 antibody

Bone sialoprotein I antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (<a href="/products/0806-6" style="font-weight: bold;text-decoration: underline;">0806-6</a>) at 1/1,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0806-6" style="font-weight: bold;text-decoration: underline;">0806-6</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (0806-6) at 1/1,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 60 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0806-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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