Osteopontin Rabbit Polyclonal Antibody

Usd: 315 Special Discount

Specification

100μl

Bulk Order Quote | Customization Request

Catalog# 0806-6

Osteopontin Rabbit Polyclonal Antibody

Application

Reactivity

Mouse

Predicted reactivity

Human
Rat

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Osteopontin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Osteopontin aa 1-314.

Species Reactivity

Mouse (Predicted: Human, Rat)

Validated Applications

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate.

Conjugation

unconjugated

RRID

AB_3068666

Reactivity Data

Tested

Verified (internally validated) Published

Reported in literature (not internally validated) Predicted

Predicted reactive (based on sequence homology) Not recommended

Not recommended (failed internal validation)

	WB
Mouse
Human
Rat

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

WB
1:500-1:2,000

Target

Function

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.

Background References

1. Han H et al. Macrophage-derived Osteopontin (SPP1) Protects From Nonalcoholic Steatohepatitis. Gastroenterology. 2023 Jul

2. Shirakawa K et al. Osteopontin in Cardiovascular Diseases. Biomolecules. 2021 Jul

Sequence Similarity

Belongs to the osteopontin family.

Post-translational Modification

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

Synonyms

BNSP antibody

Bone sialoprotein 1 antibody

Bone sialoprotein I antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

Expand

Images

☑ Cell treatment (CT)

Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (0806-6) at 1/1,000 dilution.

Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 60 kDa

Exposure time: 9 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0806-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Citation

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Urolithin A supplementation alleviates osteogenic disfunction and promotes bone fracture healing in inflammatory environments
- Journal: Food & Nutrition Research
- DOI: 10.29219/fnr.v70.13033
- IF: 4.5
- Application: WB
- Reactivity: Mouse
- Publish date: 2026 May
SPARC modulates macrophage-like phenotype transformation in VSMCs and triggers a vicious cycle
- Journal: Frontiers In Cell And Developmental Biology
- DOI: 10.3389/fcell.2026.1791698
- IF: 5.3
- Application: WB
- Reactivity: Human
- Publish date: 2026 May
Unraveling the therapeutic mechanisms of Polygonum cuspidatum in pulmonary fibrosis: Modulation of M2 macrophage polarization and glycolysis pathways
- Journal: Journal Of Ethnopharmacology
- DOI: 10.1016/j.jep.2026.121461
- IF: 5.4
- Application: WB
- Reactivity: Mouse,Human
- Publish date: 2026 Mar
Senescent BMSC-Derived Thbs1 Drives Inflammaging and Impairs Bone Regeneration by Suppressing PINK1/Parkin-Mediated Mitophagy in Macrophages
- Journal: Aging Cell
- DOI: 10.1111/acel.70575
- IF: 7.7
- Application: WB
- Reactivity: Rat
- Publish date: 2026 Jun
Targeting N1-methyladenosine modification in osteoblasts through tRNA methyltransferase 10C reverses mitochondrial dysfunction and ameliorates osteoporosis
- Journal: Signal Transduction And Targeted Therapy
- DOI: 10.1038/s41392-026-02740-2
- IF: 81.2
- Application: WB
- Reactivity: Mouse
- Publish date: 2026 Jun
Mechanism of Xiao-ai-fei Honey Ointment, a Traditional Uyghur Multi-Ingredient Medicinal Preparation, Against Cervical Cancer Based on Network Pharmacology and In Vitro Evaluation of Anti-Cancer Activity
- Journal: Pharmaceuticals
- DOI: 10.3390/ph19050686
- IF: 4.8
- Application: WB
- Reactivity: Human
- Publish date: 2026 Apr
SPP1 as a key modulator of M2 macrophage polarization promotes endometriosis progression via activation of the FAK/PI3K/AKT pathway: A bioinformatics and experimental study
- Journal: International Immunopharmacology
- DOI: 10.1016/j.intimp.2025.115563
- IF: 4.7
- Application: WB
- Reactivity: Mouse,Human
- Publish date: 2025 Sept
Dynamically programmable plant-derived scaffold coordinates alveolar bone repair cascade: Phase-adaptive integration from hemostasis to bone remodeling
- Journal: Chemical Engineering Journal
- DOI: 10.1016/j.cej.2025.168416
- IF: 13.2
- Application: WB
- Reactivity: Mouse
- Publish date: 2025 Sept
Genome editing of Spp1 by inhalable CRISPR/Cas9 formulation for treating pulmonary fibrosis
- Journal: Journal Of Controlled Release
- DOI: 10.1016/j.jconrel.2025.114424
- IF: 11.5
- Application:
- Reactivity:
- Publish date: 2025 Nov
Mussel-inspired bifunctional chimeric peptides macromolecules functionalize 3D-printed porous scaffolds for enhanced antimicrobial and osseointegration properties in bone defect repair
- Journal: International Journal Of Biological Macromolecules
- DOI: 10.1016/j.ijbiomac.2025.142668
- IF: 7.7
- Application: IF-cell
- Reactivity: Rat
- Publish date: 2025 Mar
A Novel COL12A1 Mutation Causes Oral Tissue Abnormalities by Regulating Gingival Fibroblast Function
- Journal: Oral Diseases
- DOI: 10.1111/odi.70000
- IF: 2.9
- Application: WB
- Reactivity: Human
- Publish date: 2025 Jun
SPP1 promotes malignant characteristics and drug resistance in hepatocellular carcinoma by activating fatty acid metabolic pathway
- Journal: Functional & Integrative Genomics
- DOI: 10.1007/s10142-025-01664-4
- IF: 3.1
- Application: WB
- Reactivity: Mouse,Human
- Publish date: 2025 Jul
“The role of mitogen-activated protein kinase signaling pathway in bone formation during mid-palatal suture expansion”
- Journal: Connective Tissue Research
- DOI: 10.1080/03008207.2025.2498509
- IF: 2.8
- Application: IHC-P,WB
- Reactivity: Rat
- Publish date: 2025 Apr
Macrophage-derived amphiregulin promoted the osteogenic differentiation of chondrocytes through EGFR/Yap axis and TGF-β activation
- Journal: Bone
- DOI:
- IF: 3.5
- Application: WB
- Reactivity: Mouse
- Publish date: 2024 Oct
Beta-adrenergic receptor antagonist propranolol prevents bisphosphonate-related osteonecrosis of the jaw by promoting osteogenesis
- Journal: Journal Of Dental Sciences
- DOI:
- IF: 3.5
- Application: WB
- Reactivity: Rat
- Publish date: 2024 May
Mussel-inspired immunomodulatory and osteoinductive dual-functional hydroxyapatite nanoplatform for promoting bone regeneration
- Journal: Journal Of Nanobiotechnology
- DOI:
- IF: 10.2
- Application: WB
- Reactivity: Mouse
- Publish date: 2024 Jun
MicroRNA-29c-tetrahedral framework nucleic acids: Towards osteogenic differentiation of mesenchymal stem cells and bone regeneration in critical-sized calvarial defects
- Journal: Cell Proliferation
- DOI: 10.1111/cpr.13624
- IF: 5.9
- Application: WB
- Reactivity: Rat
- Publish date: 2024 Feb
miR-181a/MSC-Loaded Nano-Hydroxyapatite/Collagen Accelerated Bone Defect Repair in Rats by Targeting Ferroptosis Pathway
- Journal: Journal Of Functional Biomaterials
- DOI:
- IF: 5
- Application: IHC-P
- Reactivity: Rat
- Publish date: 2024 Dec
A Multifunctional miRNA Delivery System Based on Tetrahedral Framework Nucleic Acids for Regulating Inflammatory Periodontal Ligament Stem Cells and Attenuating Periodontitis Bone Loss
- Journal: ACS Applied Materials & Interfaces
- DOI:
- IF: 8.3
- Application: WB
- Reactivity: Rat
- Publish date: 2024 Dec
Mettl3 regulates hypertrophic differentiation of chondrocytes through modulating Dmp1 mRNA via Ythdf1-mediated m6A modification
- Journal: Bone
- DOI:
- IF: 4.1
- Application:
- Reactivity: Mouse
- Publish date: 2022 Nov
The role of ephrinB2-EphB4 signalling in bone remodelling during orthodontic tooth movement
- Journal: Orthodontics & Craniofacial Research
- DOI: 10.1111/ocr.12591
- IF: 2.563
- Application: IHC-P
- Reactivity: Rat
- Publish date: 2022 May
Mechanisms of sphingosine-1-phosphate (S1P) signaling on excessive stress-induced root resorption during orthodontic molar intrusion
- Journal: Clinical Oral Investigations
- DOI:
- IF: 3.606
- Application: IHC-P
- Reactivity: Rat
- Publish date: 2022 Jan
Nano-Sized Hydroxyapatite Induces Apoptosis and Osteogenic Differentiation of Vascular Smooth Muscle Cells via JNK/c-JUN Pathway. International journal of nanomedicine, 16, 3633–3648.
- Journal: International Journal Of Nanomedicine
- DOI:
- IF: 4.471
- Application: WB
- Reactivity: Human
- Publish date: 2021 May
The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, 159(3), e217–e232.
- Journal: American Journal Of Orthodontics And Dentofacial Orthopedics
- DOI:
- IF: 1.98
- Application:
- Reactivity: Rat
- Publish date: 2021 Mar
Nano-hydroxyapatite accelerates vascular calcification via lysosome impairment and autophagy dysfunction in smooth muscle cells
- Journal: Bioactive Materials
- DOI:
- IF: 14.593
- Application: WB
- Reactivity: Human
- Publish date: 2021 Jun
miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38
- Journal: Stem Cell Research & Therapy
- DOI:
- IF: 6.832
- Application: WB
- Reactivity: Human
- Publish date: 2021 Jul
USP7 regulates the proliferation and differentiation of ATDC5 cells through the Sox9-PTHrP-PTH1R axis
- Journal: Bone
- DOI:
- IF: 4.40
- Application: WB
- Reactivity: Mouse
- Publish date: 2021 Feb
BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell death & disease, 12(2), 197.
- Journal: Cell Death & Disease
- DOI:
- IF: 6.304
- Application: IHC-P
- Reactivity: Mouse
- Publish date: 2021 Feb
Parathyroid hormone increases alveolar bone homoeostasis during orthodontic tooth movement in rats with periodontitis via crosstalk between STAT3 and β-catenin
- Journal: International Journal Of Oral Science
- DOI:
- IF: 3.05
- Application: WB
- Reactivity: Rat
- Publish date: 2020 Dec
Intermittent parathyroid hormone promotes cementogenesis in a PKA- and ERK1/2-dependent manner
- Journal: Journal Of Periodontology
- DOI:
- IF: 2.77
- Application: WB
- Reactivity: Mouse
- Publish date: 2019 Sept
Circulating miR-338 Cluster activities on osteoblast differentiation: Potential Diagnostic and Therapeutic Targets for Postmenopausal Osteoporosis
- Journal: Theranostics
- DOI:
- IF: 8.063
- Application: WB
- Reactivity: Mouse
- Publish date: 2019 May
CircPOMT1 and circMCM3AP inhibit osteogenic differentiation of human adipose-derived stem cells by targeting miR-6881-3p
- Journal: American Journal Of Translational Research
- DOI:
- IF: 3.27
- Application: WB
- Reactivity: Human
- Publish date: 2019 Aug
Regulation of Vascular Calcification by Growth Hormone–Releasing Hormone and Its Agonists
- Journal: Circulation Research
- DOI:
- IF: 15.211
- Application: WB
- Reactivity: Human
- Publish date: 2018 May

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Osteopontin Rabbit Polyclonal Antibody

Usd: 315 Special Discount

Specification

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Target Molecular Weight

Positive Control

Conjugation

RRID

Reactivity Data

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Sequence Similarity

Post-translational Modification

Subcellular Location

UNIPROT

Synonyms

Images

Citation

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Application: IHC-P,IF-Tissue

Reactivity: Human

Conjugate: unconjugated

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