Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23]
Overview
Product Name
Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-23]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Osteopontin aa 1-314.
Species Reactivity
Human
Validated Applications
IHC-Fr, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Human kidney tissue, human bone tissue.
Conjugation
unconjugated
Clone Number
PSH09-23
Reactivity Data
| IHC-Fr | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
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| Mouse |
|
|
|
| Rat |
|
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:200
-
IHC-P
-
1:2,000
-
IF-Tissue
-
1:200-1:500
Target
Function
Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.
Background References
1. Han H et al. Macrophage-derived Osteopontin (SPP1) Protects From Nonalcoholic Steatohepatitis. Gastroenterology. 2023 Jul
2. Shirakawa K et al. Osteopontin in Cardiovascular Diseases. Biomolecules. 2021 Jul
Subcellular Location
Secreted.
Synonyms
BNSP antibody
Bone sialoprotein 1 antibody
Bone sialoprotein I antibody
BSP I antibody
BSPI antibody
Early T lymphocyte activation 1 antibody
ETA 1 antibody
ETA1 antibody
MGC110940 antibody
Nephropontin antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Genome editing of Spp1 by inhalable CRISPR/Cas9 formulation for treating pulmonary fibrosis
Journal: Journal Of Controlled Release
DOI: 10.1016/j.jconrel.2025.114424
IF: 11.5
Application:
Reactivity:
Publish date: 2025 Nov
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