Skip to content

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA724629

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Application

  • WB

  • IHC-P

  • FC(Intra)

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Osteoprotegerin aa 1-401.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC(Intra), IHC-Fr

Target Molecular Weight

Predicted band size: 46 kDa

Positive Control

K-562 (Human chronic myelogenous leukemia cell) cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH26-52

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P IHC-Fr FC(Intra) IF-Cell
human
Tested Tested
Not Recommended
 
Tested Tested
Not Recommended
mouse
Tested Tested
Tested Tested
Tested Tested
Not Recommended
Not Recommended
rat
Tested Tested
Tested Tested
Tested Tested
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

  • FC(Intra)

  • 1:1,000

  • IHC-Fr

  • 1:500

Target

Function

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) or tumour necrosis factor receptor superfamily member 11B (TNFRSF11B), is a cytokine receptor of the tumour necrosis factor (TNF) receptor superfamily encoded by the TNFRSF11B gene. OPG was first discovered as a novel secreted TNFR related protein that played a role in the regulation of bone density and later for its role as a decoy receptor for receptor activator of nuclear factor kappa-B ligand (RANKL). OPG also binds to TNF-related apoptosis-inducing ligand (TRAIL) and inhibits TRAIL induced apoptosis of specific cells, including tumour cells. Other OPG ligands include syndecan-1, glycosaminoglycans, von Willebrand factor, and factor VIII-von Willebrand factor complex. OPG has been identified as having a role in tumour growth and metastasis, heart disease, immune system development and signalling, mental health, diabetes, and the prevention of pre-eclampsia and osteoporosis during pregnancy.

Background References

1. Dutka M et al. Osteoprotegerin and RANKL-RANK-OPG-TRAIL signalling axis in heart failure and other cardiovascular diseases. Heart Fail Rev. 2022 Jul

2. Xu M et al. Association between Osteoprotegerin rs2073618 polymorphism and peri-implantitis susceptibility: a meta-analysis. BMC Oral Health. 2022 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: O00300 Human

SwissProt: O08712 Mouse

SwissProt: O08727 Rat

Synonyms

MGC29565 antibody

OCIF antibody

OPG antibody

Osteoclastogenesis inhibitory factor antibody

Osteoprotegerin antibody

PDB5 antibody

TNF receptor superfamily member 11b antibody

TNFRSF 11B antibody

TNFRSF11B antibody

TR 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (<a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>) at 1/5,000 dilution. <br /> <br />Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate <br />Lane 2: Mouse liver tissue lysate <br />Lane 3: Mouse kidney tissue lysate <br />Lane 4: Mouse skeletal muscle tissue lysate <br />Lane 5: Rat liver tissue lysate <br />Lane 6: Rat kidney tissue lysate <br />Lane 7: Rat skeletal muscle tissue lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 46 kDa <br />Observed band size: 56 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (HA724629) at 1/5,000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Mouse skeletal muscle tissue lysate
    Lane 5: Rat liver tissue lysate
    Lane 6: Rat kidney tissue lysate
    Lane 7: Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724629, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 46 kDa
    Observed band size: 56 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Skeletal muscle <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of Osteoprotegerin protein in skeletal muscle is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Skeletal muscle
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of Osteoprotegerin protein in skeletal muscle is consistent with the predicted expression pattern.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: knee joint<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/200, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: knee joint
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: knee joint<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/200, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: knee joint
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Human<br />Sample: K-562 (Human chronic myelogenous leukemia cell)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: K-562 (Human chronic myelogenous leukemia cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724629 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Kidney<br />Sample: Frozen section<br /><br />Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br /><br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Kidney
    Sample: Frozen section

    Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA724629, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Rat<br />Tissue: Kidney<br />Sample: Frozen section<br /><br />Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br /><br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Kidney
    Sample: Frozen section

    Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA724629, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52] - BSA and Azide free

Application: WB,IHC-P,FC(Intra),IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Osteoprotegerin Mouse Monoclonal Antibody [9C2]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Osteoprotegerin Mouse Monoclonal Antibody [9C1]

Application: WB,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Osteoprotegerin Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Human Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH13-32] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated

metafield image

Biotin Conjugated Human Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH13-32] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

Human Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH13-31] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated