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P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]

Usd: 385

Specification

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Catalog# HA724296

P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]

Application

  • WB

Reactivity

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse P2X7 aa 354-595.

Species Reactivity

Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 68 kDa

Positive Control

Mouse brain lysate, Mouse lung tissue lysate.

Conjugation

unconjugated

Clone Number

PSH22-44

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:25,000

Target

Function

P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purinoceptors for ATP. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay criteria. The receptor is found in the central and peripheral nervous systems, in microglia, in macrophages, in uterine endometrium, and in the retina. The P2X7 receptor also serves as a pattern recognition receptor for extracellular ATP-mediated apoptotic cell death, regulation of receptor trafficking, mast cell degranulation, and inflammation. Regarding inflammation, P2X7 receptor induces the NLRP3 inflammasome in myeloid cells and leads to interleukin-1beta release.

Background References

1. Pegoraro A et al. P2X7 Variants in Pathophysiology. Int J Mol Sci. 2024 Jun

2. De Marchi E et al. P2X7 Receptor in Hematological Malignancies. Front Cell Dev Biol. 2021 Mar

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9Z1M0 Mouse

Synonyms

ATP receptor antibody

P2rx7 antibody

P2X purinoceptor 7 antibody

P2X7 antibody

P2Z receptor antibody

Purinergic receptor antibody

purinergic receptor P2X, ligand gated ion channel, 7 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (<a href="/products/HA724296" style="font-weight: bold;text-decoration: underline;">HA724296</a>) at 1/25,000 dilution.<br /><br />Lane 1: Mouse brain lysate (no heat)<br />Lane 2: Mouse skeletal muscle lysate (no heat) (negative)<br />Lane 3: Mouse lung tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724296" style="font-weight: bold;text-decoration: underline;">HA724296</a>) at 1/25,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br /><br />Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881)

    ☑ Relative expression (RE)

    Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (HA724296) at 1/25,000 dilution.

    Lane 1: Mouse brain lysate (no heat)
    Lane 2: Mouse skeletal muscle lysate (no heat) (negative)
    Lane 3: Mouse lung tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 75 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724296) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

    Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"