PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric)
Catalog# HA601677
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric)
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human PARK7 aa 11-60 / 189.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
SN07-21
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:1,000
Target
Function
PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies. Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.
Background References
1. Marín-Buera L et al. Differential proteomic profiling unveils new molecular mechanisms associated with mitochondrial complex III deficiency. J Proteomics 113:38-56 (2015).
2. Bifsha P et al. Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet 10:e1004863 (2014).
Subcellular Location
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus.
Synonyms
CAP1 antibody
DJ-1 antibody
DJ1 antibody
DJ1 protein antibody
Epididymis secretory sperm binding protein Li 67p antibody
FLJ27376 antibody
FLJ34360 antibody
FLJ92274 antibody
HEL S 67p antibody
Oncogene DJ1 antibody
ExpandImages
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Western blot analysis of PARK7/DJ1 on different lysates with Rat anti-PARK7/DJ1 antibody (HA601677) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601677, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
PARK7/DJ1 Recombinant Rabbit Monoclonal Antibody [SN07-21] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
