Skip to content

PD1 Mouse Monoclonal Antibody

Usd: 278

Specification

Bulk Order Quote | Customization Request

Catalog# IRS190MS

PD1 Mouse Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PD1 Mouse Monoclonal Antibody

Antibody Type

Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

mIHC

Molecular Weight

Predicted band size: 32 kDa

Positive Control

Human tonsil tissue, human spleen tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Application Dilution

  • mIHC

  • 1:100

Target

Function

Programmed cell death protein 1 (PD-1), (CD279 cluster of differentiation 279). PD-1 is a protein encoded in humans by the PDCD1 gene. PD-1 is a cell surface receptor on T cells and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells). PD-1 inhibitors, a new class of drugs that block PD-1, activate the immune system to attack tumors and are used to treat certain types of cancer. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. PD-1 binds two ligands, PD-L1 and PD-L2.

Background References

1. Lei Q et al. Resistance Mechanisms of Anti-PD1/PDL1 Therapy in Solid Tumors. Front Cell Dev Biol. 2020 Jul

2. Chamoto K et al. Insights from a 30-year journey: function, regulation and therapeutic modulation of PD1. Nat Rev Immunol. 2023 Oct

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q15116 Human

Synonyms

CD279 antibody

CD279 antigen antibody

hPD 1 antibody

hPD l antibody

hPD-1 antibody

hSLE1 antibody

PD 1 antibody

PD-1 antibody

PD1 antibody

PDCD 1 antibody

Expand

Images

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Human PD1 Recombinant Rabbit Monoclonal Antibody [PSH13-39] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated

metafield image

PD1 Recombinant Rabbit Monoclonal Antibody [SJ01-91]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

Human PD1 Recombinant Rabbit Monoclonal Antibody [PSH13-38] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated

metafield image

PD1 Recombinant Rabbit Monoclonal Antibody [SJ01-91] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

Biotin Conjugated Human PD1 Recombinant Rabbit Monoclonal Antibody [PSH13-38] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

Biotin Conjugated Human PD1 Recombinant Rabbit Monoclonal Antibody [PSH13-39] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

Human PD1 Recombinant Rabbit Monoclonal Antibody [SJ01-91] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated

metafield image

PD1 Mouse Monoclonal Antibody [1F2]

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated