PKC zeta Recombinant Rabbit Monoclonal Antibody [PSH06-74] - BSA and Azide free
Catalog# HA751122
PKC zeta Recombinant Rabbit Monoclonal Antibody [PSH06-74] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
PKC zeta Recombinant Rabbit Monoclonal Antibody [PSH06-74] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PKC zeta aa 201-250.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 68 kDa
Positive Control
293T cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, A431 cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-74
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Protein kinase C, zeta (PKCζ), also known as PRKCZ, is a protein in humans that is encoded by the PRKCZ gene. The PRKCZ gene encodes at least two alternative transcripts, the full-length PKCζ and an N-terminal truncated form PKMζ. PKMζ is thought to be responsible for maintaining long-term memories in the brain. Atypical PKC (aPKC) isoforms [zeta (this enzyme) and lambda/iota] play important roles in insulin-stimulated glucose transport. Human adipocytes contain PKC-zeta, rather than PKC-lambda/iota, as their major aPKC. Inhibition of the PKCζ enzyme inhibits insulin-stimulated glucose transport while activation of PKCζ increases glucose transport.
Background References
1. Cao YJ et al. PKC-zeta Aggravates Doxorubicin-Induced Cardiotoxicity by Inhibiting Wnt/beta-Catenin Signaling. Front Pharmacol. 2022 Feb
2. Zhang F et al. PKC-zeta mediated reduction of the extracellular vesicles-associated TGF-beta1 overcomes radiotherapy resistance in breast cancer. Breast Cancer Res. 2023 Apr
Subcellular Location
Cytoplasm, Endosome, Cell junction, Membrane.
UNIPROT
Synonyms
14-3-3-zetaisoform antibody
AI098070 antibody
aPKCzeta antibody
C80388 antibody
EC 2.7.11.13 antibody
KPCZ_HUMAN antibody
nPKC zeta antibody
nPKC-zeta antibody
OTTHUMP00000001368 antibody
OTTHUMP00000044160 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA722773, the same antibody clone in a different buffer formulation.
Western blot analysis of PKC zeta on different lysates with Rabbit anti-PKC zeta antibody (HA722773) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HL-60 cell lysate (negative)
Lane 3: HeLa cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 74 kDa
Exposure time: 1 minute 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722773) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PKC zeta Recombinant Rabbit Monoclonal Antibody [PSH06-74]
Application: WB
Reactivity: Human
Conjugate: unconjugated
