HIV-1 lentiviral BCA protein assay kit
Catalog# PQ01
HIV-1 lentiviral BCA protein assay kit
Application
-
ELISA
Reactivity
-
Human
Overview
Product Name
HIV-1 lentiviral BCA protein assay kit
Product Type
BCA protein assay kit
Sensitivity
| μg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.1039 | 0.1039 | 0.1039 | |
| 10.00 | 0.1196 | 0.1190 | 0.1193 | 0.0154 |
| 25.00 | 0.1424 | 0.1430 | 0.1427 | 0.0388 |
| 75.00 | 0.2098 | 0.2094 | 0.2096 | 0.1057 |
| 200.00 | 0.3519 | 0.3540 | 0.3530 | 0.2491 |
| 500.00 | 0.6408 | 0.6380 | 0.6394 | 0.5355 |
| 1000.00 | 1.0423 | 1.0500 | 1.0462 | 0.9423 |
| 2000.00 | 1.7229 | 1.7210 | 1.7220 | 1.6181 |
Precision
Spike Recovery
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
Standard Curve Gradient
Incubation Count
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Function
The BCA method is a method of judging the concentration of a protein by showing the color change of a sample at different concentrations. In the alkaline environment it provides, the protein can reduce the Cu2+ in the BCA reagent to Cu+, and the Cu+ can then be chelated with the BCA reagent to produce a blue-purple complex with a strong absorbance peak at 562 nm wavelength, and the protein concentration can be known by measuring the absorbance and combining it with the standard curve.
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