Peroxiredoxin 2 Recombinant Mouse Monoclonal Antibody [7F2-R] - BSA and Azide free
Catalog# HA610123
Peroxiredoxin 2 Recombinant Mouse Monoclonal Antibody [7F2-R] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
Peroxiredoxin 2 Recombinant Mouse Monoclonal Antibody [7F2-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 22 kDa
Positive Control
HEK-293 cell lysate, LNCaP cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, HepG2 cell lysate.
Conjugation
unconjugated
Clone Number
7F2-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
Background References
1. Kang S W et al. Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha. J Biol Chem 273:6297-6302 (1998).
2. Kamariah N et al. Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins. Sci Rep 6:37610-37610 (2016).
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Epididymis secretory sperm binding protein Li 2a antibody
HEL S 2a antibody
MGC4104 antibody
Natural killer cell enhancing factor B antibody
Natural killer cell-enhancing factor B antibody
Natural Killer Enhancing Factor B antibody
NKEF B antibody
NKEF-B antibody
NKEFB antibody
Peroxiredoxin-2 antibody
ExpandImages
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This data was developed using HA601248, the same antibody clone in a different buffer formulation.
Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (HA601248) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HeLa cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601248) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA601248, the same antibody clone in a different buffer formulation.
Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (HA601248) at 1/20,000 dilution.
Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si Peroxiredoxin 2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601248) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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