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Peroxiredoxin 2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1510-37

Peroxiredoxin 2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Peroxiredoxin 2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human PRDX2 aa 149-198 / 198.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 22 kDa

Positive Control

HeLa cell lysate, PC-12 cell lysate, A549 cell lysate, 293T cell lysate, mouse heart tissue lysate, Hela, SHG-44, SH-SY5Y, human liver tissue, human kidney tissue, mouse brain tissue, mouse prostate tissue, mouse heart tissue.

Conjugation

unconjugated

RRID

AB_3073345

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

Target

Function

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization。The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.

Background References

暂无

Sequence Similarity

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Post-translational Modification

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P32119 Human

SwissProt: Q61171 Mouse

SwissProt: P35704 Rat

Synonyms

Epididymis secretory sperm binding protein Li 2a antibody

HEL S 2a antibody

MGC4104 antibody

Natural killer cell enhancing factor B antibody

Natural killer cell-enhancing factor B antibody

Natural Killer Enhancing Factor B antibody

NKEF B antibody

NKEF-B antibody

NKEFB antibody

Peroxiredoxin-2 antibody

Expand

Images

  • Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: PC-12 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: 293T cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 15 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 15 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 2 minutes;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: Mouse heart tissue lysate (20 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 2 minutes;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si Peroxiredoxin 2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1510-37" style="font-weight: bold;text-decoration: underline;">R1510-37</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/5,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si Peroxiredoxin 2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining Peroxiredoxin 2/PRDX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Peroxiredoxin 2/PRDX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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