Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18] - BSA and Azide free
Catalog# HA750524
Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18] - BSA and Azide free
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr1989 of human ATR.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 301 kDa
Positive Control
HeLa cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours.
Conjugation
unconjugated
Clone Number
PS01-18
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:200
Target
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex.
Background References
1. Ma M et al. Activation of ATR-related protein kinase upon DNA damage recognition. Curr Genet. 2020 Apr
2. Tiernan H et al. ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals. Spectrochim Acta A Mol Biomol Spectrosc. 2020 Nov
Subcellular Location
Chromosome, Nucleus.
UNIPROT
Synonyms
Ataxia telangiectasia and Rad3 related antibody
Ataxia telangiectasia and Rad3-related protein antibody
ATR antibody
ATR_HUMAN antibody
FCTCS antibody
FRAP Related Protein 1 antibody
FRAP-related protein 1 antibody
FRP1 antibody
MEC1 antibody
MEC1 mitosis entry checkpoint 1 homolog antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with Hydroxyurea cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750524) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells treated with or without 4mM Hydroxyurea for 20 hours labeling Phospho-ATR (T1989) with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/200 dilution. Image shown an increased nuclear staining upon Hydroxyurea treatment.
Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS permeabilization for 5 minutes, and then blocked with 2% negative goat serum for 60 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/200 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18]
Application: WB,IF-Cell
Reactivity: Human
Conjugate: unconjugated
ATR Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
