Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody [PSH10-28]
Catalog# HA723195
Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody [PSH10-28]
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WB
-
Mouse
-
Rat
-
Human
-
unconjugated
Overview
Product Name
Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody [PSH10-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser660 of Human HSL.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 117 kDa
Positive Control
Mouse white adipose tissue lysate, Rat white adipose tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-28
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Rat |
|
| Human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Hormone-sensitive lipase (EC 3.1.1.79, HSL), also previously known as cholesteryl ester hydrolase (CEH), sometimes referred to as triacylglycerol lipase, is an enzyme that, in humans, is encoded by the LIPE gene. HSL is an intracellular neutral lipase capable of hydrolyzing a variety of esters. The enzyme has a long and a short form. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Background References
1. Shen WJ et al. Hormone sensitive lipase ablation promotes bone regeneration. Biochim Biophys Acta Mol Basis Dis. 2022 Sep
2. Heier C et al. Hormone-sensitive lipase couples intergenerational sterol metabolism to reproductive success. Elife. 2021 Feb
Subcellular Location
Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet.
Synonyms
Hormone sensitive lipase antibody
Hormone sensitive lipase testicular isoform antibody
Hormone-sensitive lipase antibody
HSL antibody
LHS antibody
Lipase hormone sensitive antibody
LIPE antibody
LIPS_HUMAN antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody (HA723195) at 1/2,000 dilution.
Lane 1: Mouse white adipose tissue lysate
Lane 2: Rat white adipose tissue lysate
Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 40 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 84 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody [PSH10-28] - BSA and Azide free
Application: WB
Reactivity: Mouse,Rat,Human
Conjugate: unconjugated
