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Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]

Usd: 385

Specification

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Catalog# HA721513

Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 106 kDa

Positive Control

Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate.

Conjugation

unconjugated

Clone Number

JE44-61

RRID

AB_3072629

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The retinoblastoma protein (protein name abbreviated Rb; gene name abbreviated Rb, RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, inactivating it, and the cell cycle is allowed to progress. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomavirus, bind and inactivate pRb, this can lead to cancer. The RB gene may have been responsible for the evolution of multicellularity in several lineages of life including animals.

Background References

1. Linn P et al. Targeting RB1 Loss in Cancers. Cancers (Basel). 2021 Jul

2. Yao Y et al. Novel insights into RB1 mutation. Cancer Lett. 2022 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P06400 Human

SwissProt: P13405 Mouse

SwissProt: P33568 Rat

Synonyms

Exon 17 tumor GOS561 substitution mutation causes premature stop antibody

GOS563 exon 17 substitution mutation causes premature stop antibody

OSRC antibody

Osteosarcoma antibody

p105-Rb antibody

P105RB antibody

PP105 antibody

pp110 antibody

PPP1R130 antibody

pRb antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (<a href="/products/HA721513" style="font-weight: bold;text-decoration: underline;">HA721513</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat serum starved for 44 hours whole cell lysate<br />Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate<br />Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate<br />Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 106 kDa<br />Observed band size: 106 kDa<br /><br />Exposure time: 1 minute 59 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721513" style="font-weight: bold;text-decoration: underline;">HA721513</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.

    Lane 1: Jurkat serum starved for 44 hours whole cell lysate
    Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate
    Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate
    Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

    Exposure time: 1 minute 59 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Phospho-Rb (S780) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Rb (S780) antibody (<a href="/products/HA721513" style="font-weight: bold;text-decoration: underline;">HA721513</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 106 kDa<br />Observed band size: 106 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721513" style="font-weight: bold;text-decoration: underline;">HA721513</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-Rb (S780) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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