NANOG Rabbit Polyclonal Antibody (R1108-2)
Catalog# R1108-2
Rabbit Polyclonal to NANOG
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WB
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Human
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Mouse
Lane 1: NCCIT cell lysate
Lane 2: JAR cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1108-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
NANOG Rabbit Polyclonal Antibody (R1108-2)
Immunogen
Synthetic peptide within mouse nanog aa 56-100.
Host
Rabbit
Positive Control
NCCIT cell lysate, JAR cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NANOG
SYNONYMS
Embryonic stem cell specific homeobox protein (Nanog) antibody;ENK antibody;FLJ12581 antibody;hNanog antibody;Homeobox protein NANOG antibody;Homeobox transcription factor Nanog antibody;homeobox transcription factor Nanog-delta 48 antibody;NANOG antibody;Nanog homeobox antibody;NANOG_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the Nanog homeobox family.
TISSUE SPECIFICITY
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
DEVELOPMENTAL STAGE
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
SUBCELLULAR LOCATION
Nucleus
FUNCTION
NANOG is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. NANOG is thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. Overexpression of NANOG in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent.
CITATIONS
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Sun Fen,et al
Albumin-Based MUC13 Peptide Nanomedicine Suppresses Liver Cancer Stem Cells via JNK-ERK Signaling Pathway-Mediated Autophagy Inhibition
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WB
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Mouse
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Citation
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