PRMT5 Rabbit Polyclonal Antibody
Catalog# R1204-3
PRMT5 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
PRMT5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Prtm5.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Molecular Weight
73kDa
Positive Control
HepG2, NCCIT.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50
Target
Function
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. PRMT5 is a highly conserved arginine methyltransferase that translocated from the cytoplasm to the nucleus at embryonic day ~E8.5, and during preimplantation development at the ~4-cell stage. Model organisms have been used in the study of PRMT5 function. A conditional knockout mouse line, called Prmt5tm2a(EUCOMM)Wtsi was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.
Background References
1. Kwak, Youn Tae; Guo Jun, Prajapati Shashi, Park Kyu-Jin, Surabhi Rama M, Miller Brady, Gehrig Peter, Gaynor Richard B (Apr. 2003). "Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties". Mol. Cell (United States) 11 (4): 1055–66.
2. Hong E, Lim Y, Lee E, Oh M, Kwon D. “Tissue-specific and age-dependent expression of protein arginine methyltransferases (PRMTs) in male rat tissues”. Biogerontology. 2012 Apr 7.
3. Lim JH, Choi YJ, Cho CH, Park JW.Protein arginine methyltransferase 5 is an essential component of the hypoxia-inducible factor 1 signaling pathway. Biochem Biophys Res Commun. 2012 Feb 10; 418(2):254-9.
Sequence Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
Tissue Specificity
Ubiquitous.
Subcellular Location
Cytoplasm, nucleus
Synonyms
72 kDa ICln binding protein antibody
72 kDa ICln-binding protein antibody
ANM5_HUMAN antibody
Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
Histone-arginine N-methyltransferase PRMT5 antibody
HMT1 hnRNP methyltransferase like 5 antibody
HOMOLOG OF
SKB1 antibody
HRMT1L5 antibody
IBP72 antibody
ExpandImages
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Western blot analysis of PRMT5 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1204-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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ICC staining of PSMA3 in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-3, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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