beta Actin Rabbit Polyclonal Antibody (R1207-1)
Catalog# R1207-1
Rabbit Polyclonal to beta Actin
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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Zebrafish
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Bamboo
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lane 8: Mouse lung tissue lysate
Lane 9: Rat lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1207-1) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
beta Actin Rabbit Polyclonal Antibody (R1207-1)
Immunogen
Synthetic peptide within n-terminal residues of β-actin.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, HeLa, NIH/3T3, PC-12, human kidney tissue, human lung tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue, hybrid fish (crucian-carp) brain tissue, hybrid fish (crucian-carp) kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:10,000
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IF-Cell
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1:100
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IHC-P
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1:2,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
beta Actin
SYNONYMS
A26C1A antibody;A26C1B antibody;ACTB antibody;ACTB_HUMAN antibody;Actin beta antibody;Actin cytoplasmic 1 antibody;Actin, cytoplasmic 1, N-terminally processed antibody;Actx antibody;b actin antibody;Beta cytoskeletal actin antibody;Beta-actin antibody;BRWS1 antibody;E430023M04Rik antibody;MGC128179 antibody;PS1TP5 binding protein 1 antibody;PS1TP5BP1 antibody;β-actin antibody;β actin antibody
SEQUENCE SIMILARITIES
Belongs to the actin family.
POST-TRANSLATIONAL MODIFICATION
ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
SUBCELLULAR LOCATION
Cytoskeleton
FUNCTION
Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.
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