CD24 Rabbit Polyclonal Antibody
Catalog# R1303-1
CD24 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
CD24 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human CD24 aa 1-50 / 80.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 8 kDa
Positive Control
MCF7 cell lysates, human colon carcinoma tissue.
Conjugation
unconjugated
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:50-1:200
Target
Function
Signal transducer CD24 also known as cluster of differentiation 24 or heat stable antigen CD24 (HSA) is a protein that in humans is encoded by the CD24 gene. CD24 is a cell adhesion molecule. CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. The protein also contributes to a wide range of downstream signaling networks and is crucial for neural development. Cross-linking of CD24 on the surface of neutrophils induces apoptosis, and this appears to be defective in sepsis. CD24 gene is found on chromosome 6 (6q21) An alignment of this gene's sequence finds genomic locations with similarity on chromosomes 1p36, 3p26, 15q21.3, 20q11.2 and Yq11.222.
Background References
1. Kay R, Rosten PM, Humphries RK. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol 1991; 147: 1412-1416
2. Aigner S, Ruppert M, Hubbe M, Sammar M, Sthoeger Z, Butcher EC, Vestweber D, Altevogt P. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol 1995; 7: 1557-1565
Sequence Similarity
Belongs to the CD24 family.
Tissue Specificity
B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Post-translational Modification
Extensively O-glycosylated.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 24 antibody
CD24 antibody
CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
CD24 antigen antibody
CD24 molecule antibody
CD24_HUMAN antibody
CD24A antibody
FLJ22950 antibody
FLJ43543 antibody
GPI linked surface mucin antibody
ExpandImages
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Western blot analysis of CD24 on MCF7 cell lysates with Rabbit anti-CD24 antibody (R1303-1) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 8 kDa
Observed band size: 45 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1303-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD24 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1303-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD24 Recombinant Rabbit Monoclonal Antibody [PSH10-85]
Application: IF-Cell,FC,WB
Reactivity: Human
Conjugate: unconjugated
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