Stella Rabbit Polyclonal Antibody (R1306-1)
Catalog# R1306-1
Rabbit Polyclonal to Stella
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Zebrafish
Western blot analysis of Stella on different lysates with Rabbit anti-Stella antibody (R1306-1) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: F9 cell lysate
Lane 4: NCCIT cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: F9 cell lysate
Lane 4: NCCIT cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
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