Stella Rabbit Polyclonal Antibody
Catalog# R1306-1
Stella Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Zebrafish
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unconjugated
Overview
Product Name
Stella Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse Stella aa 101-150 / 150 (ID:Q8QZY).
Species Reactivity
Human, Mouse (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, F9 cell lysate, NCCIT cell lysate, mouse teratoma tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:100-1:200
Target
Function
Stella is a primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. It participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. It is important for the totipotent/pluripotent states continuing through preimplantation development.
Background References
1. "Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma." Clark A.T., Rodriguez R.T., Bodnar M.S., Abeyta M.J., Cedars M.I., Turek P.J., Firpo M.T., Reijo Pera R.A. Stem Cells 22:169-179(2004)
2. "Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours." Bowles J., Teasdale R.P., James K., Koopman P. Cytogenet. Genome Res. 101:261-265(2003)
Tissue Specificity
Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
Subcellular Location
Cell nucleus, cytoplasm
Synonyms
Compaction associated protein 1 antibody
Developmental pluripotency associated 3 antibody
Developmental pluripotency associated protein 3 antibody
Developmental pluripotency-associated protein 3 antibody
Dppa3 antibody
DPPA3_HUMAN antibody
PGC7 antibody
Stella related protein antibody
Stella-related protein antibody
Images
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Western blot analysis of Stella on different lysates with Rabbit anti-Stella antibody (R1306-1) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: F9 cell lysate
Lane 4: NCCIT cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse teratoma tissue using anti-Stella rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"