DPY30 Rabbit Polyclonal Antibody
Catalog# R1312-4
DPY30 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
DPY30 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Full length protein.
Species Reactivity
Human, Mouse
Validated Applications
IF-Cell, IHC-P
Molecular Weight
Predicted band size: 11kDa
Positive Control
NCCIT , F9, D3, human lung carcinoma tissue, mouse brain tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:100-1:200
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IF-Cell
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1:100-1:200
Target
Function
As part of the MLL1/MLL complex, DPY30 is involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. DPY30 may play some role in histone H3 acetylation. In a teratocarcinoma cell, it plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. DPY30 may also play an indirect or direct role in endosomal transport. In the recent study, it showed that depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage.
Background References
1. "Crystal structure of the C-terminal domain of human DPY-30-like protein: A component of the histone methyltransferase complex." Wang X., Lou Z., Dong X., Yang W., Peng Y., Yin B., Gong Y., Yuan J., Zhou W., Bartlam M., Peng X., Rao Z. J. Mol. Biol. 390:530-537(2009)
2. "A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking." Xu Z., Gong Q., Xia B., Groves B., Zimmermann M., Mugler C., Mu D., Matsumoto B., Seaman M., Ma D. J. Cell Biol. 186:343-353(2009)
3. "Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains." Jiang H., Shukla A., Wang X., Chen W.Y., Bernstein B.E., Roeder R.G. Cell 144:513-525(2011)
Sequence Similarity
Belongs to the dpy-30 family.
Subcellular Location
Nucleus. Golgi apparatus
Synonyms
Dpy-30-like protein antibody
Dpy-30L antibody
DPY30 antibody
DPY30_HUMAN antibody
HDPY-30 antibody
Protein dpy-30 homolog antibody
Saf19 antibody
Images
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Immunocytochemistry analysis of NCCIT cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of F9 cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- DPY30 rabbit polyclonal antibody.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPY30 rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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