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CRISPR-Cas9 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1403-1

CRISPR-Cas9 Rabbit Polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Staphylococcus aureus

Conjugation

  • unconjugated

Overview

Product Name

CRISPR-Cas9 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within StreptococcusThermophilus CRISPR-Cas9 aa 374-415/1388.

Species Reactivity

Staphylococcus aureus

Validated Applications

WB, ELISA

Positive Control

Cas9 transfected 293 cells

Conjugation

unconjugated

RRID

AB_3073290

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:10,000

Target

Function

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

Background References

1. Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12.

2. Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.

UNIPROT

SwissProt: Q03JI6 StreptococcusThermophilus

Synonyms

Cas9 antibody

CRISPR-associated endonuclease Cas9/Csn1 antibody

CRISPR-Cas9/Csn1 antibody

csn1 antibody

SpyCas9 antibody

Images

  • Western blot analysis of CRISPR-Cas9 on different lysates with Rabbit anti-CRISPR-Cas9 antibody (<a href="/products/R1403-1" style="font-weight: bold;text-decoration: underline;">R1403-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293-WT cell lysate<br />Lane 2: 293T-OE cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Exposure time: 180 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1403-1" style="font-weight: bold;text-decoration: underline;">R1403-1</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Observed band size: 158kDa

    Western blot analysis of CRISPR-Cas9 on different lysates with Rabbit anti-CRISPR-Cas9 antibody (R1403-1) at 1/1,000 dilution.

    Lane 1: 293-WT cell lysate
    Lane 2: 293T-OE cell lysate

    Lysates/proteins at 20 µg/Lane.

    Exposure time: 180 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1403-1, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Observed band size: 158kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"