CUGBP Elav-like family member 2 Rabbit Polyclonal Antibody
Catalog# R1403-3
CUGBP Elav-like family member 2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CUGBP Elav-like family member 2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Human CUGBP Elav-like family member 2 aa450-508 / 508.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 54 kDa
Positive Control
PC-3M cell lysate, Jurkat cell lysate, THP-1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, Hela, MCF-7, SHG-44.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
Target
Function
Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawl.
Background References
1. Gazzara MR et al. Ancient antagonism between CELF and RBFOX families tunes mRNA splicing outcomes. Genome Res 27(8):1360-1370 (2017).
2. Schultz AS et al. Activation-Dependent TRAF3 Exon 8 Alternative Splicing Is Controlled by CELF2 and hnRNP C Binding to an Upstream Intronic Element. Mol Cell Biol 37(7) pii: e00488-16 (2017).
Sequence Similarity
Belongs to the CELF/BRUNOL family.
Tissue Specificity
Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
Subcellular Location
Nucleus. Cytoplasm.
Synonyms
Bruno-like protein 3 antibody
BRUNOL3 antibody
Cardiac elav type RNA binding protein antibody
CELF-2 antibody
celf2 antibody
CELF2_HUMAN antibody
CUG BP2 antibody
CUG triplet repeat RNA-binding protein 2 antibody
CUG-BP- and ETR-3-like factor 2 antibody
CUG-BP2 antibody
ExpandImages
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Western blot analysis of CUGBP Elav-like family member 2 on different lysates with Rabbit anti-CUGBP Elav-like family member 2 antibody (R1403-3) at 1/1,000 dilution.
Lane 1: PC-3M cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: THP-1 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Rat brain tissue lysate (30 µg/Lane)
Lane 6: Rat cerebellum tissue lysate (30 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1403-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
ICC staining CELF2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining CELF2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining CELF2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"