Psg16 Rabbit Polyclonal Antibody
Catalog# R1408-5
Psg16 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Mouse
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unconjugated
Overview
Product Name
Psg16 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse Psg16 aa 346-395 / 395.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
Mouse brain, D3
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100-1:200
Target
Function
The murine pregnancy-specific glycoprotein-16 (PSG16; previously brain carcinoembryonic antigen/bCEA) is expressed in the brain, especially in neurons, and has previously been reported to be an MHV receptor. The murine pregnancy-specific glycoproteins (PSGs), which mediate placentation and modulate interactions between the fetus and the maternal immune system, resemble the related carcinoembryonic antigen (CEA) proteins in their extracellular Ig-like domains but are secreted rather than membrane-anchored.
Background References
1. Chen DS, Asanaka M, Yokomori K, Wang F, Hwang SB, Li HP, Lai MM. A pregnancy-specific glycoprotein is expressed in the brain and serves as a receptor for mouse hepatitis virus. Proc Natl Acad Sci U S A. 1995; 92:12095–9.
2. McLellan AS, Fischer B, Dveksler G, Hori T, Wynne F, Ball M, Okumura K, Moore T, Zimmermann W. Structure and evolution of the mouse pregnancy-specific glycoprotein (Psg) gene locus. BMC Genomics. 2005; 6:4.
Subcellular Location
Cell membrane, Cell projection
UNIPROT
Synonyms
Pregnancy specific glycoprotein 16, Pregnancy-specific glycoprotein 16
Images
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ICC staining Psg16 in mouse embryonic stem cells D3 (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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Western blot analysis of Psg16 on different lysates with Rabbit anti-Psg16 antibody (R1408-5) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1408-5, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 45 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"