Gamma tubulin Rabbit Polyclonal Antibody
Catalog# R1411-2
Gamma tubulin Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Gamma tubulin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human Gamma tubulin.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 51 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human heart tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1: 1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:100
Target
Function
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
Background References
1. Chumová J. et. al. Microtubular and Nuclear Functions of gamma-Tubulin: Are They LINCed? Cells. 2019 Mar
2. Tovey CA. et. al. Microtubule nucleation by gamma-tubulin complexes and beyond. Essays Biochem. 2018 Dec
Sequence Similarity
Belongs to the tubulin family.
Post-translational Modification
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Subcellular Location
Cytoplasm, Cytoskeleton, Microtubule.
Synonyms
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP-1 antibody
GCP1 antibody
MGC131994 antibody
ExpandImages
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Western blot analysis of Gamma tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1411-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Human heart tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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