5 Lipoxygenase Rabbit Polyclonal Antibody

Function

5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells. 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis. The cellular localization of 5-LO varies between cell types. In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope. In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane. This intracellular shuttling of 5-LO is dependent on the association with various signaling molecules, phosphorylation and the presence of a distinct

Background References

1. Li C et al. Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism. Sci Rep 6:36949 (2016).

2. Fredman G et al. An imbalance between specialized pro-resolving lipid mediators and pro-inflammatory leukotrienes promotes instability of atherosclerotic plaques. Nat Commun 7:12859 (2016).

Sequence Similarity

Belongs to the lipoxygenase family.

Post-translational Modification

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-524 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Phosphorylation at Ser-524 is stimulated by 8-bromo-3',5'-cyclic AMP or prostaglandin E2.

Subcellular Location

Cytoplasm, Membrane, Nucleus.

UNIPROT

SwissProt: P09917 Human

SwissProt: P48999 Mouse

SwissProt: P12527 Rat

Synonyms