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GPC1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1512-15

GPC1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

GPC1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding toHuman GPC1 aa 1-50 / 558.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 61 kDa

Positive Control

Hela cell lysate, SK-Br-3 cell lysate, Hela, MCF-7, PANC-1, human breast tissue, human breast cancer tissue.

Conjugation

unconjugated

RRID

AB_3073391

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Glypican-1 (GPC1), a member of the glycosylphosphatidilinositol anchored cell surface heparan sulfate proteoglycans, is involved with cell adhesion and migration, lipoprotein metabolism, modulation of growth factor activites and anticoagulation. Glypican-1 binds to and modulates the activity of several fibroblast growth factors (FGFs) including FGF-1, FGF-2 and FGF-7. Glypican-1 acts as an extracellular chaperone for VEGF165 to help restore receptor binding ability after oxidation. The heparan sulfate chains of glypican-1 mediate specific binding of glypican-1 to VEGF165. When present on the surface of marrow stromal cells, glypican-1 may aid in the maintenance and development of hematopoietic stem and progenitor cells. Human pancreatic cancer cells express a large amount of glypican-1 when compared to glypican-1 levels in normal pancreatic cells. Glypican-1 may play an important role in the response of pancreatic cancer cells to mitogenic stimuli, such as FGF-2. The gene encoding human glypican-1 maps to chromosome 2q37.3.

Background References

1. Cave C et al. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord. Mol Neurodegener 12:8 (2017).

2. Sharma P et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015).

Sequence Similarity

Belongs to the glypican family.

Post-translational Modification

S-nitrosylated in a Cu(2+)-dependent manner. Nitric acid (NO) is released from the nitrosylated cysteines by ascorbate or by some other reducing agent, in a Cu(2+) or Zn(2+) dependent manner. This free nitric oxide is then capable of cleaving the heparan sulfate side chains.; N- and O-glycosylated. N-glycosylation is mainly of the complex type containing sialic acid. O-glycosylated with heparan sulfate. The heparan sulfate chains can be cleaved either by the action of heparanase or, degraded by a deaminative process that uses nitric oxide (NO) released from the S-nitrosylated cysteines. This process is triggered by ascorbate, or by some other reducing agent, in a Cu(2+)- or Zn(2+) dependent manner. Cu(2+) ions are provided by ceruloproteins such as APP, PRNP or CP which associate with GCP1 in intracellular compartments or lipid rafts.; This cell-associated glypican is further processed to give rise to a medium-released species.

Subcellular Location

Cell membrane, Endosome, Membrane, Secreted.

UNIPROT

SwissProt: P35052 Human

Synonyms

FLJ38078 antibody

glypican antibody

Glypican proteoglycan 1 antibody

glypican1 antibody

GPC 1 antibody

Gpc1 antibody

GPC1_HUMAN antibody

Secreted glypican-1 antibody

Images

  • Western blot analysis of GPC1 on MCF7cell lysate with Rabbit anti-GPC1 antibody (<a href="/products/R1512-15" style="font-weight: bold;text-decoration: underline;">R1512-15</a>) at 1/1,000 dilution.<br /><br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1512-15" style="font-weight: bold;text-decoration: underline;">R1512-15</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 61.7 kDa<br />Observed band size: 61.7 kDa

    Western blot analysis of GPC1 on MCF7cell lysate with Rabbit anti-GPC1 antibody (R1512-15) at 1/1,000 dilution.

    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1512-15, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 61.7 kDa
    Observed band size: 61.7 kDa

  • ICC staining Glypican-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glypican-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Glypican-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glypican-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Glypican-1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glypican-1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"