Neurofilament Rabbit Polyclonal Antibody

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilament-H (NF-H), also known as neurofilament heavy polypeptide, and Neurofilament-L (NF-L), also known as neurofilament light polypeptide, members of the intermediate filament family, are major components of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.

Background References

1. Medina-Rodruez EM et al. Promoting in vivo remyelination with small molecules: a neuroreparative pharmacological treatment for Multiple Sclerosis. Sci Rep 7:43545 (2017).

2. Vasconcelos DM et al. Immune response and innervation signatures in aseptic hip implant loosening. J Transl Med 14:205 (2016).

Sequence Similarity

Belongs to the intermediate filament family.

Post-translational Modification

There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.; Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Subcellular Location

Cytoplasm, Intermediate filament.

UNIPROT

SwissProt: P12036 Human

SwissProt: P19246 Mouse

SwissProt: P16884 Rat

Synonyms

200 kDa neurofilament protein antibody

CMT2CC antibody

Nefh antibody

Neurofilament heavy polypeptide 200kDa antibody

Neurofilament heavy polypeptide antibody

Neurofilament triplet H protein antibody

NF H antibody

NF-H antibody

NFH antibody

NFH_HUMAN antibody