RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78] - BSA and Azide free
Catalog# HA750972
RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78] - BSA and Azide free
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WB
-
IHC-P
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Human
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HA722182
含抗保成分
-
unconjugated
Overview
Product Name
RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human RIP3 aa 401-450 / 518.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 57 kDa
Positive Control
THP-1 cell lysate, HT-29 cell lysate, SK-Br-3 cell lysate, HT-29, human colon cancer tissue.
Conjugation
unconjugated
Clone Number
PSH04-78
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IHC-P
-
1:2,000-1:5,000
Target
Function
Receptor-interacting serine/threonine-protein kinase 3 is an enzyme that is encoded by the RIPK3 gene in humans. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. It contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce necroptosis by interaction with RIPK1 and MLKL in a protein complex termed the necrosome. Interactions between RIPK1 and RIPK3 also form a necrosome, which triggers apoptosis.
Background References
1. Zeng X et al. Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis. Mil Med Res. 2022 May
2. Chen X et al. Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. Nat Cell Biol. 2022 Apr
Subcellular Location
Cytoplasm, cytosol, Nucleus.
UNIPROT
Synonyms
Receptor interacting protein 3 antibody
Receptor interacting serine threonine kinase 3 antibody
Receptor interacting serine/threonine protein kinase 3 antibody
Receptor-interacting protein 3 antibody
Receptor-interacting serine/threonine-protein kinase 3 antibody
RIP 3 antibody
RIP like protein kinase 3 antibody
RIP-3 antibody
RIP-like protein kinase 3 antibody
RIPK 3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (HA750972) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate (30 µg/Lane)
Lane 2: HT-29 cell lysate (30 µg/Lane)
Lane 3: SK-Br-3 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (negative) (30 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (HA750972) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750972) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (HA750972) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750972) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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