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RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-79] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750973

RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-79] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-79] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human RIP3 aa 469-518 / 518.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

THP-1 cell lysate, HT-29 cell lysate, SK-Br-3 cell lysate, HT-29, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

PSH04-79

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Receptor-interacting serine/threonine-protein kinase 3 is an enzyme that is encoded by the RIPK3 gene in humans. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. It contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce necroptosis by interaction with RIPK1 and MLKL in a protein complex termed the necrosome. Interactions between RIPK1 and RIPK3 also form a necrosome, which triggers apoptosis.

Background References

1. Zeng X et al. Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis. Mil Med Res. 2022 May

2. Chen X et al. Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. Nat Cell Biol. 2022 Apr

Subcellular Location

Cytoplasm, cytosol, Nucleus.

UNIPROT

SwissProt: Q9Y572 Human

Synonyms

Receptor interacting protein 3 antibody

Receptor interacting serine threonine kinase 3 antibody

Receptor interacting serine/threonine protein kinase 3 antibody

Receptor-interacting protein 3 antibody

Receptor-interacting serine/threonine-protein kinase 3 antibody

RIP 3 antibody

RIP like protein kinase 3 antibody

RIP-3 antibody

RIP-like protein kinase 3 antibody

RIPK 3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (30 µg/Lane)<br />Lane 2: HT-29 cell lysate (30 µg/Lane)<br />Lane 3: SK-Br-3 cell lysate (30 µg/Lane)<br />Lane 4: HeLa cell lysate (negative) (30 µg/Lane)<br /><br />Predicted band size: 57 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (HA750973) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate (30 µg/Lane)
    Lane 2: HT-29 cell lysate (30 µg/Lane)
    Lane 3: SK-Br-3 cell lysate (30 µg/Lane)
    Lane 4: HeLa cell lysate (negative) (30 µg/Lane)

    Predicted band size: 57 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (HA750973) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750973) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750973" style="font-weight: bold;text-decoration: underline;">HA750973</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (HA750973) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750973) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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