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PPAR gamma Mouse Monoclonal Antibody [E-8]

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Catalog# RT1668

PPAR gamma Mouse Monoclonal Antibody [E-8]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PPAR gamma Mouse Monoclonal Antibody [E-8]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

specific for an epitope mapping between amino acids 486-505 at the C-terminus of PPARγ of human origin (identical to corresponding mouse sequence)

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 58 kDa

Positive Control

Jurkat cell lysate, U-937 cell lysate, THP-1 cell lysate, Mouse ovary tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

E-8

Product Features

Form

Liquid

Concentration

0.2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 4℃.

Storage Buffer

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Isotype

IgG1

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:1,000

Target

Function

Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor subfamily of transcription factors, form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARg is implicated in diseases including obesity, diabetes, atherosclerosis and cancer. PPARg activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. PPARg is a key component in adipocyte differentiation and fat-specific gene expression. A Pro12Ala polymorphism of the PPARg2 gene may reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARg2 gene may be correlated with abdominal obesity in type 2 diabetes.

Background References

1. Chen H et al. PPAR-gamma signaling in nonalcoholic fatty liver disease: Pathogenesis and therapeutic targets. Pharmacol Ther. 2023 May

2. Stark JM et al. The Role of PPAR-gamma in Allergic Disease. Curr Allergy Asthma Rep. 2021 Oct

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P37231 Human

SwissProt: P37238 Mouse

SwissProt: O88275 Rat

Synonyms

CIMT1 antibody

GLM1 antibody

NR1C3 antibody

Nuclear receptor subfamily 1 group C member 3 antibody

OTTHUMP00000185032 antibody

OTTHUMP00000185036 antibody

Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody

Peroxisome proliferator activated receptor gamma 1 antibody

Peroxisome Proliferator Activated Receptor gamma antibody

Peroxisome proliferator-activated receptor gamma antibody

Expand

Images

  • Western blot analysis of PPAR gamma on different lysates with Mouse anti-PPAR gamma antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: U-937 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: Mouse ovary tissue lysate<br />Lane 5: Mouse liver tissue lysate<br />Lane 6: Rat liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 58/50 kDa<br /><br />Exposure time: 59 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PPAR gamma on different lysates with Mouse anti-PPAR gamma antibody (RT1668) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: U-937 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: Mouse ovary tissue lysate
    Lane 5: Mouse liver tissue lysate
    Lane 6: Rat liver tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 58/50 kDa

    Exposure time: 59 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (RT1668) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-PPAR gamma antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-PPAR gamma antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/RT1668" style="font-weight: bold;text-decoration: underline;">RT1668</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation