AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
Catalog# ET7109-45
AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human AP-A aa 860-957 / 957.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 109 kDa
Positive Control
Human kidney tissue lysate, human small intestine tissue lysate, human kidney tissue, human small intestine tissue.
Conjugation
unconjugated
Clone Number
JE46-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,000
Target
Function
Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. ENPEP is considered to be an essential and highly specific enzyme, which metabolizes and inactivates bioactive peptides such as angiotensin II (AngII) in the renin-angiotensin system. ENPEP is expressed in neoplastic lesions of the uterine cervix, and its levels are upregulated as the lesions progress from cervical intraepithelial neoplasms to invasive squamous cell carcinomas. ENPEP expression is absent in normal cells and is overexpressed in cancer cell lines compared with normal cells of mammary origin.
Background References
1. Yang Y et al. Structural insights into central hypertension regulation by human aminopeptidase A. J Biol Chem 288:25638-25645 (2013).
2. Ino K et al. Expression of aminopeptidase A in human gestational choriocarcinoma cell lines and tissues. Placenta 21:63-72 (2000).
Sequence Similarity
Belongs to the peptidase M1 family.
Tissue Specificity
Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Subcellular Location
Cell membrane, Membrane.
UNIPROT
Synonyms
Aminopeptidase A antibody
AMPE_HUMAN antibody
AP-A antibody
APA antibody
bp-1 antibody
bp1 antibody
CD249 antibody
Differentiation antigen gp160 antibody
EAP antibody
Enpep antibody
ExpandImages
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Western blot analysis of AP-A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human kidney tissue lysate
Lane 2: Human small intestine tissue lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"