ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36]
Catalog# ET7110-29
ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ERAP1 aa 833-941 / 941.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 107 kDa
Positive Control
A431 cell lysate, PC-12 cell lysate, Mouse heart tissue lysate, Rat liver tissue lysate, human placenta tissue.
Conjugation
unconjugated
Clone Number
JE52-36
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. ERAP1 is also designated adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). Peptides presented by MHC class I on the surface of a cell must be eight to eleven residues long and ERAP1 specifically trims peptides of nine amino acids or more. ERAP1 is induced by interferon-g and encoded for by the ARTS1 gene, which maps to human chromosome 5q15. ERAP1 is thought to inactivate several bioactive peptides, including angioten-sin II, and subsequently, may be involved in the regulation of blood pressure. It may have a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and is characterized as a TNFR1 binding protein that promotes TNFR1 shedding.
Background References
1. Vitulano C. et. al. The interplay between HLA-B27 and ERAP1/ERAP2 aminopeptidases: from anti-viral protection to spondyloarthritis. Clin Exp Immunol. 2017 Dec;190(3):281-290.
2. Roberts AR. et. al. ERAP1 association with ankylosing spondylitis is attributable to common genotypes rather than rare haplotype combinations. Proc Natl Acad Sci U S A. 2017 Jan 17;114(3):558-561.
Sequence Similarity
Belongs to the peptidase M1 family.
Tissue Specificity
Ubiquitous.
Post-translational Modification
N-glycosylated.
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
A LAP antibody
A-LAP antibody
Adipocyte derived leucine aminopeptidase antibody
Adipocyte-derived leucine aminopeptidase antibody
ALAP antibody
Aminopeptidase PILS antibody
Aminopeptidase regulator of TNFR1 shedding antibody
APPILS antibody
ARTS 1 antibody
ARTS-1 antibody
ExpandImages
-
☑ Knockout (KO)
All lanes: Western blot analysis of ERAP1 with anti-ERAP1 antibody [JE52-36] (ET7110-29) at 1/500 dilution.
Lane 1: Wild-type 293T whole cell lysate.
Lane 2: ERAP1 knockout 293T whole cell lysate.
ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). -
Western blot analysis of ERAP1 on different lysates with Rabbit anti-ERAP1 antibody (ET7110-29) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ERAP1 antibody (ET7110-29) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"