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P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]

Usd: 385

Specification

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Catalog# ET7108-52

P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]

Application

  • WB

  • IP

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human P2Y6 aa 279-328 / 328.

Species Reactivity

Human

Validated Applications

WB, IP, IHC-P, IF-Cell

Molecular Weight

36 kDa

Positive Control

SK-Br-3, A549, human liver tissue, human colon tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

JG37-77

RRID

AB_3070850

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:50

Target

Function

The product of this gene, P2Y6, belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene.

Background References

1. Communi D et al. Cloning, functional expression and tissue distribution of the human P2Y6 receptor. Biochemical and Biophysical Research Communications 222(2): 303-8 (1996).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q15077 Human

Synonyms

G coupled nucleotide receptor antibody

G protein coupled 6 antibody

MGC15335 antibody

P2 purinoceptor antibody

P2ry6 antibody

P2RY6_HUMAN antibody

P2Y purinoceptor 6 antibody

P2Y6 antibody

P2Y6 receptor antibody

PP2891 antibody

Expand

Images

  • Western blot analysis of P2Y6 on different lysates with Rabbit anti-P2Y6 antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/1,000 dilution.<br /><br />Line 1: SK-Br-3 cell lysates <br />Line 2: A549 cell lysates <br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of P2Y6 on different lysates with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/1,000 dilution.

    Line 1: SK-Br-3 cell lysates
    Line 2: A549 cell lysates

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-P2Y6 antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-P2Y6 antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-P2Y6 antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-52" style="font-weight: bold;text-decoration: underline;">ET7108-52</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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