Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
Catalog# ET7108-13
Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
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WB
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IHC-P
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FC
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IP
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IF-Cell
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IF-Tissue
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Human
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Mouse
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unconjugated
Overview
Product Name
Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Pumilio 1 aa 79-223 .
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, FC, IP, IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 126 kDa
Positive Control
SiHa cell lysate, 293T, SiHa, human colon tissue, human appendix tissue, mouse kidney tissue, mouse small intestine tissue.
Conjugation
unconjugated
Clone Number
JG37-75
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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1:10-1:50
Target
Function
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.
Background References
1. Lee S et al. Noncoding RNA NORAD regulates genomic stability by sequestering PUMILIO proteins. Cell 164:69-80 (2016).
2. Narita R et al. A novel function of human Pumilio proteins in cytoplasmic sensing of viral infection. PLoS Pathog 10:E1004417-E1004417 (2014).
Tissue Specificity
Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
Post-translational Modification
Phosphorylation at Ser-714 promotes RNA-binding activity. Following growth factor stimulation phosphorylated at Ser-714, promoting binding to the 3'-UTR of CDKN1B/p27 mRNA.
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
HsPUM antibody
KIAA0099 antibody
PUM 1 antibody
PUM L1 antibody
Pum1 antibody
PUM1_HUMAN antibody
PUMH 1 antibody
PUMH antibody
PUMH1 antibody
pumilio homolog 1 (Drosophila) antibody
ExpandImages
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Western blot analysis of Pumilio 1 on SiHa cell lysate using anti-Pumilio 1 antibody at 1/1,000 dilution.
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ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"