SOX18 Recombinant Rabbit Monoclonal Antibody [ST43-02]
Catalog# ET1609-11
SOX18 Recombinant Rabbit Monoclonal Antibody [ST43-02]
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WB
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
SOX18 Recombinant Rabbit Monoclonal Antibody [ST43-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SOX18 aa 250-384 / 384.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB
Molecular Weight
Predicted band size: 41 kDa
Positive Control
K562 cell lysates.
Conjugation
unconjugated
Clone Number
ST43-02
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. Sox-18 is a 384 amino acid nuclear protein that contains one HMG box DNA-binding domain and belongs to the Sox family of transcriptional regulators.
Background References
1. Petrovic, I. et al. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. PloS one 10: e0143591 (2015).
2. Petrovic, I. et al. ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. Molecular biology reports 36: 993-1000 (2009).
Tissue Specificity
Detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, msosmall intestine and colon.
Subcellular Location
Nucleus.
Synonyms
HLTS antibody
SOX 18 antibody
Sox18 antibody
SOX18_HUMAN antibody
SRY (sex determining region Y) box 18 antibody
SRY box 18 antibody
Transcription factor SOX 18 antibody
Transcription factor SOX-18 antibody
Images
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Western blot analysis of SOX18 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"