SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]
Catalog# ET1701-62
SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]
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WB
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IF-Cell
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SUZ12 aa 1-150/739.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IP
Molecular Weight
Predicted band size: 83 kDa
Positive Control
MCF-7 cells lysates, NIH/3T3 cell lysate, PC-12 cell lysate, Raw264.7 cell lysate, SW480, MCF-7, Hela, SKOV-3.
Conjugation
unconjugated
Clone Number
JJ09-04
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis. The mammalian PcG proteins are regulatory proteins important for Hox gene expression, axial skeleton development and the control of proliferation and survival of hematopoietic cells. By inducing changes in chromatin structure, the PcG proteins are part of a cellular memory system that is responsible for gene activity being inherited to progeny cells. PcG proteins silence gene expression through the formation of multimeric protein complexes with different compositions. Manipulating the expression-levels of various PcG proteins in mammalian cell lines results in cellular transformation, which may be a link between the chromatin-associated PcG proteins and cancer. Polycomb protein SUZ12, also designated ChET 9 protein or joined-to-JAZF1 protein, is a nuclear protein belonging to the VEFS (VRN2-EMF2-FIS2-SUZ12) family. SUZ12 has been detected at the breakpoints of a certain recurrent chromosomal translocation which has been reported in endometrial stromal sarcoma. It is a component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7.
Background References
1. Heubach J et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer 14:108 (2015).
2. Negishi M et al. A New lncRNA, APTR, Associates with and Represses the CDKN1A/p21 Promoter by Recruiting Polycomb Proteins. PLoS One 9:e95216 (2014).
Sequence Similarity
Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
Tissue Specificity
Overexpressed in breast and colon cancer.
Post-translational Modification
Sumoylated, probably by PIAS2.
Subcellular Location
Nucleus.
Synonyms
ChET 9 protein antibody
CHET9 antibody
Chromatin precipitated E2F target 9 protein antibody
JJAZ1 antibody
Joined to JAZF1 protein antibody
KIAA0160 antibody
Polycomb protein SUZ12 antibody
Suppressor of zeste 12 homolog antibody
Suppressor of zeste 12 protein homolog antibody
SUZ12 antibody
ExpandImages
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Western blot analysis of SUZ12 on different lysates with Rabbit anti-SUZ12 antibody (ET1701-62) at 1/500 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Raw264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.
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IF-cell staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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IF-cell staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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IF-cell staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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IF-cell staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"